SWC Advanced Microscopy Facility

Specs

  • Excitation: Mai Tai eHP DS (usable range ~760 to 1040 nm)
  • Laser modulation: Conoptics Pockels cell
  • Detection: Four PMT channels. Hamamatsu R10699 multialkali.
  • Objective: Nikon 16× NA 0.8 water immersion
  • Scanhead: 8 kHz resonant scanner optically coupled to galvo
  • Optical sectioning: PIFOC
  • Vibratome: Leica VT1000

Filters

Dichroic pass bands

  • FF635-Di01 — long pass, splits red from far red.
  • DMSP-490R — short pass, splits green from blue.

Bandpass filters

Chan #"Colour"Filter namePass bandPMT serial number
1Far red Brightline 676/29 700–661 nmAFK5930
2Red Chroma 605/70m 640–570 nm AFK5929
3Green Brightline 525/39 550–505 nm AFK6125
4Blue Brightline basic 460/60 491–437 nm AFK6121
Transmission spectra of the four BrainSaw 1 bandpass filters, spanning roughly 425 to 700 nm
Transmission of the four installed bandpass filters.