NeuroVision BrainSaw
NeuroVision was a TissueCyte 1000 (TissueVision) serial section 2-photon that was converted to BakingTray in 2019. Along with the change in acquisition software, the original open collection path was replaced with a more compact, closed, path. Other upgrades included an 8 kHz resonant scanner, motorised coarse Z, and a Leica VT1000 vibratome. The original lift stage and controller were eventually replaced with a more compact unit from Aerotech and, in 2026, NeuroVision's multi-alkali PMTs were upgraded to SiPM detectors. Like our other BrainSaw microscopes, NeuroVision's water bath clamps the slide in a consistent position across runs, simplifying setup.
Specs
- Excitation: Mai Tai eHP DS (usable range ~760 to 1040 nm)
- Laser modulation: Conoptics Pockels cell
- Detection: Switch to Thorlabs PDA45 SiPMs at Q2 2026. Prior: Hamamatsu R10699 PMTs.
- Objective: Nikon 16× NA 0.8 (max FOV 1.2 mm on a side)
- Optical sectioning: PIFOC
- Scanhead: optically coupled 8 kHz reso/galvo
- Vibratome: Leica VT1000
Filters
| Chan # | "Name" | Filter |
|---|---|---|
| 1 | Empty | — |
| 2 | Red | ET570lp (570 nm to about 700 nm) installed 27/11/2019 |
| 3 | Green | ET525/50m (~500 to 550 nm) installed 20/11/2019 |
| 4 | Blue | FF01-450/70 installed 20/11/2019 |
