SWC Advanced Microscopy Facility

Specs

  • Excitation: Mai Tai eHP DS (usable range ~760 to 1040 nm) and Coherent Axon-1064.
  • Laser modulation: Conoptics Pockels cell
  • Detection: Four PMT channels. Hamamatsu R10699 multialkali.
  • Objective: Nikon 16× NA 0.8 water immersion
  • Optical sectioning: PIFOC
  • Scanhead: 4 kHz resonant scanner optically coupled to galvo
  • Vibratome: Leica VT1000

Filters

Dichroic pass bands

Bandpass filters

Chan #"Colour"Filter range (nominal)FilterPMT serial number
1Far red~700–635 nm Chroma ET 670/50
2Red635–565 nm Semrock 607/70
3Green565–495 nm Semrock 525/50 AFT5332
4Blue<495 nm ThorLabs 460-60 AFT5458
Transmission spectra of the four OrganGrinder bandpass filters, spanning roughly 425 to 700 nm
Transmission of the four installed bandpass filters.