OrganGrinder BrainSaw
Specs
- Excitation: Mai Tai eHP DS (usable range ~760 to 1040 nm) and Coherent Axon-1064.
- Laser modulation: Conoptics Pockels cell
- Detection: Four PMT channels. Hamamatsu R10699 multialkali.
- Objective: Nikon 16× NA 0.8 water immersion
- Optical sectioning: PIFOC
- Scanhead: 4 kHz resonant scanner optically coupled to galvo
- Vibratome: Leica VT1000
Filters
Dichroic pass bands
- Main low pass: FF562-Di03-25x36
- Long wavelength portion: ThorLabs DMLP638R low pass
- Short wavelength portion: ThorLabs DMSP490R short pass
Bandpass filters
| Chan # | "Colour" | Filter range (nominal) | Filter | PMT serial number |
|---|---|---|---|---|
| 1 | Far red | ~700–635 nm | Chroma ET 670/50 | — |
| 2 | Red | 635–565 nm | Semrock 607/70 | — |
| 3 | Green | 565–495 nm | Semrock 525/50 | AFT5332 |
| 4 | Blue | <495 nm | ThorLabs 460-60 | AFT5458 |